Journal: Physiological Research
Article Title: Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro
doi: 10.33549/physiolres.934692
Figure Lengend Snippet: Release of 17β-estradiol (A) and progesterone (B) by human ovarian granulosa cells HGL5 after treatment with isoquercitrin (5, 10, 25, 50, 100 μg.ml−1). Control represents culture medium without isoquercitrin; positive control (+Control) means culture medium enriched by 0.1 % DMSO. Significance of differences between the groups was evaluated by One-way ANOVA followed by Dunnett’s multiple comparison tests. The data are expressed as means ± SEM. ELISA.
Article Snippet: Concentrations of secreted 17β-estradiol, progesterone, TGF-β2 and TGF-β2 receptor were determined using ELISA kit (CUSABIO, Houston, USA) as described previously ( Michalcova et al. 2019 , Baldovská et al. 2020 ).
Techniques: Control, Positive Control, Comparison, Enzyme-linked Immunosorbent Assay